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. 2021 Nov 30;12:6994. doi: 10.1038/s41467-021-27076-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — The central lines indicate median with interquartile range as error bars, and each point on the graph represents the mean of a sample run in duplicate. False negative samples were recorded as time to positive reaction at 45 min. Statistical analysis was performed using a one-sided non-parametric Kruskal-Wallis with Dunn’s multiple comparisons test (one-sided). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. a HCV RNA samples detection based on genotype (Gt): Gt1 (grey square, n = 26), Gt2 (white and black disc, n = 14), Gt3 (grey triangle, n = 23), Gt4 (grey lozenge, n = 22), Gt5 (black disc, n = 3), Unknown (grey inverse triangle, n = 10). Gt1 vs Gt3, p < 0.0001, Gt1 vs Gt4, p < 0.0001, Gt1 vs Unknown, p = 0.1524, Gt2 vs Gt3, p = 0.0101, Gt2 vs Gt4, p = 0.0284, Gt3 vs Gt5, p = 0.1846, Gt4 vs Gt5, p = 0.2814. All remaining groups had p values of >0.9999. b HCV cDNA samples detection based on genotype; Gt1 (grey square, n = 26), Gt2 (white and black disc, n = 14), Gt3 (grey triangle, n = 23), Gt4 (grey lozenge, n = 22), Gt5 (black disc, n = 3), Unknown (grey inverse triangle, n = 10). Gt1 vs Gt3, p < 0.0001, Gt1 vs Gt4, p = 0.0562, Gt1 vs Unknown, p = 0.3425, Gt2 vs Gt3, p = 0.0002, Gt3 vs Gt4, p = 0.0490, Gt3 vs Gt5, p = 0.0035, Gt3 vs Unknown, p = 0.3247, Gt4 vs Gt5, p = 0.3701, Gt5 vs Unknown, p = 0.5210. All the remaining groups had p values of >0.9999. c HCV RNA samples detection based on viral load: 1.7–3.95 (grey square, n = 5), 4.4–4.85 (black and white disc, n = 11), 5.2–5.98 (grey triangle, n = 34), 6.05–6.97 (grey lozenge, n = 44). 1.7–3.95 vs 5.2–5.98, p = 0.1937, 1.7–3.95 vs 6.05–6.97, p = 0.0095, 4.4–4.85 vs 6.05–6.97, p = 0.0301, 5.2–5.98 vs 6.05–6.97, p = 0.4768, 5.2–5.98 vs Unknown, p = 0.4506, 6.05–6.97 vs Unknown, p = 0.0118. All remaining groups had p values of >0.9999. d HCV cDNA samples detection based on viral load; 1.7–3.95 (grey square, n = 5), 4.4–4.85 (black and white disc, n = 11), 5.2–5.98 (grey triangle, n = 34), 6.05–6.97 (grey lozenge, n = 44). 1.7–3.95 vs 5.2–5.98, p = 0.0536, 1.7–3.95 vs 6.05–6.97, p = 0.0032, 1.7–3.95 vs Unknown, p = 0.5693, 4.4–4.85 vs 6.05–6.97, p = 0.0492, 5.2–5.98 vs 6.05–6.97, p = 0.9431, 6.05–6.97 vs Unknown, p = 0.7858. All remaining groups had p values of >0.9999. Source data are provided as a Source data file.