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. 2021 Nov 30;12:6996. doi: 10.1038/s41467-021-27344-y

Fig. 4. WFS1 traffics from the ER to the Golgi.

Fig. 4

a WFS1 distributes in the ER and Golgi fractions. Lysates from INS1 cells were subjected to sucrose gradient centrifugation and IB. ER fractions were marked by calnexin and Golgi fractions were marked by GM130. Molecular weights are in kDa. n = 3 independent experiments. b–e Confocal microscopy analysis of colocalization of WFS1-GFP and ER-Dsred (KDEL, ER marker) in HEK-293T cells treated with normal (37 °C) or low temperature (30 °C) (b). Trace outline is used for line-scan (white dashed line) analysis of the relative fluorescence intensity of WFS1 and ER signals treated with normal (37 °C, c) or low temperature (30 °C, d). Signal overlap was quantified by Pearson correlation analysis of n = 3 independent experiments (e). Scale bar, 5 μm. f–i Confocal microscopy analysis of colocalization of WFS1-GFP and Golgi-TDimer2 (Golgi marker) in HEK-293T cells treated with normal (37 °C) or low temperature (30 °C) (f). Trace outline is used for line-scan (white dashed line) analysis of the relative fluorescence intensity of WFS1 and Golgi signals treated with normal (37 °C, g) or low temperature (30 °C, h). Signal overlap was quantified by Pearson correlation analysis of n = 3 independent experiments (i). Scale bar, 5 μm. All the data are presented as mean ± s.e.m. p < 0.05, significant, using a two-tailed Student’s t-test. Source data are provided as a Source Data file.