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. 2021 Nov 30;12:6996. doi: 10.1038/s41467-021-27344-y

Fig. 7. WFS1 recognizes vesicular cargo proteins via its luminal C-terminal segment.

Fig. 7

a The interaction between different mutant WFS1 proteins and vesicular cargo proteins evaluated by BiFC and then quantified by flow cytometry. n = 5 independent experiments. b Representative imaging of the interaction between different mutant WFS1 and vesicular cargo protein SCG5 by PLA. The puncta number per cell was quantified by ImageJ software. n = 4 independent experiments, n = 8 independent images quantified. Scale bar, 10 μm. c A model of WFS1 mediating vesicular cargo protein transport from the ER. All the data are presented as mean ± s.e.m. p < 0.05, significant, using a two-tailed Student’s t-test. Source data are provided as a Source Data file.