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. 2021 Nov 30;6:409. doi: 10.1038/s41392-021-00805-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — SKOR1 interacts with SMAD6 in adipocytes and hepatocytes. a Protein interaction prediction map of SKOR1. b, c The effect of sh-SKOR1 or Flag-SKOR1 on SMAD6 gene expression in 3T3-L1 adipocytes (b) and AML12 cells (c). d Western blot analysis of SMAD6 expression in 3T3-L1 adipocytes and AML12 cells with SKOR1 knockdown or overexpression. e Co-IP experiment of SKOR1 and SMAD6 in 3T3-L1 adipocytes and AML12 cells. Anti-SKOR1 and Anti-SMAD6 reciprocal IPs were blotted with antibodies against SKOR1 and SMAD6. Experiments were performed at least in triplicate, and the results are shown as the mean ± s.d. Student’s t-test was used to analyze the data. (*P < 0.05; **P < 0.01; ***P < 0.001)