Fig. 1. Upregulation of Lp-PLA2 in Ang II-infused mice.
C57BL/6J mice were infused with Ang II (1500 ng·kg−1·min−1) or saline for 7 days before euthanasia. The hearts and plasma of these mice were collected for analysis. a RNA-seq analysis of mouse hearts. Volcano plot showing fold change and statistical probability value of PLA2 genes and four housekeeping genes (Gapdh, Eif5, Actb, and Nono) (n = 3; fold change ≥ 2 and probability ≥ 0.8 were considered statistically significant; black triangle, significantly increased PLA2 genes; white triangle, significantly decreased PLA2 genes; gray square, unchanged PLA2 genes; empty circle, control housekeeping genes). b RT-PCR analysis of the mRNA expression of Pla2g7 in mouse heart (n = 5). c Immunohistochemical staining of Lp-PLA2 in heart tissues and quantification (n = 5; scale bar = 200 μm). d Analysis of plasma Lp-PLA2 activity (n = 7). e Double immunofluorescence analysis of macrophage (Mac-2, red) and Lp-PLA2 (green) expression in hearts. Hoechst nuclear staining is shown in blue. Three independent experiments were performed. Scale bar = 25 μm. Data are presented as mean ± standard deviation, and n represents the number of animals. ***P < 0.001.