Fig. 5. RNA-seq analysis of mouse cardiac tissues.
C57BL/6J mice received darapladib (50 mg·kg−1·d−1) or vehicle by gavage and were infused with saline or Ang II (1500 ng·kg−1·min−1) for 7 days. Hearts were collected for RNA-seq analysis. a Heatmap of differentially expressed genes that were both significantly changed in Ang II (compared to saline) and darapladib+Ang II (compared to Ang II) groups. Each column represents an individual replicate, with three replicates per group. Each row represents an individual gene. The top of the heatmap is the cluster of genes that are upregulated in Ang II-infused hearts compared with saline-infused hearts and those that are downregulated in darpladib + Ang II hearts, whereas the bottom is the cluster of genes that are downregulated in Ang II-infused hearts compared with saline-infused hearts and those that are upregulated in darapladib + Ang II hearts. The color bar represents the relative gene expression of log-transformed and normalized fragments per kilobase of transcript per million mapped reads (FPKM). Upregulated genes are shown in red, and downregulated genes are shown in blue. b Volcano plot shows the fold change and the significance of genes that were altered in darapladib+Ang II hearts vs. Ang II hearts. Upregulated genes are plotted in red, downregulated genes are plotted in blue, and genes not significant are plotted in gray. GO analysis of biological processes for the genes upregulated in the Ang II group compared with the saline group (c), and the genes downregulated in the darapladib+Ang II group compared with the Ang II group (d). Significantly changed ontology terms are mainly involved in inflammation, proliferation, fibrosis, and several fibrotic signaling pathways (c, d). KEGG pathway analysis of upregulated genes in the Ang II group compared with the saline group (e) and downregulated genes in the darapladib + Ang II group compared with the Ang II group (f). Significantly changed signaling pathways are mainly involved in inflammation, proliferation, and fibrosis (e, f). NS indicates not significant. P adj < 0.05 is considered to be different.