Fig. 1. SlMYB72 interacts with the zinc-finger homeodomain transcription factor SlZHD17.

a Protein–protein interaction between SlMYB72 and SlZHD17 in tobacco (Nicotiana benthamiana) leaves by bimolecular fluorescent complementation (BiFC) assay. Bar = 25 μm. b Interaction between SlMYB72 and the SlZHD17 protein in tobacco leaves by firefly luciferase complementation imaging (LCI) assay. c Sequence alignment analysis of SlZHD17, AtZHD2 (also named AtHB-22 and AT4G24660), and AtZHD5 (also named AtHB-33 and AT1G75240). White letters with a black background indicate identical amino acids, and the highly conserved zinc-finger domain and the DNA-binding homeodomain are boxed. The detailed amino acid sequences are provided in Appendices S2 and S3. d SlZHD17 homodimerization based on BiFC assay in tobacco leaves. Bar = 25 μm. e SlZHD17 forms a homodimer as assessed by LCI assay