TABLE 1.

Nucleotide sequences, gene locations, and anticipated sizes of PCR products for the S. aureus gene-specific oligonucleotide primers used in this study

Genea	Primer	Oligonucleotide sequence (5′-3′)	Location within gene	Size of amplified product (bp)	Multiplex PCR set
sea	GSEAR-1	GGTTATCAATGTGCGGGTGG	349–368	102	A
	GSEAR-2	CGGCACTTTTTTCTCTTCGG	431–450
seb	GSEBR-1	GTATGGTGGTGTAACTGAGC	666–685	164	A
	GSEBR-2	CCAAATAGTGACGAGTTAGG	810–829
sec	GSECR-1	AGATGAAGTAGTTGATGTGTATGG	432–455	451	A
	GSECR-2	CACACTTTTAGAATCAACCG	863–882
sed	GSEDR-1	CCAATAATAGGAGAAAATAAAAG	492–514	278	A
	GSEDR-2	ATTGGTATTTTTTTTCGTTC	750–769
see	GSEER-1	AGGTTTTTTCACAGGTCATCC	237–257	209	A
	GSEER-2	CTTTTTTTTCTTCGGTCAATC	425–445
femA	GFEMAR-1	AAAAAAGCACATAACAAGCG	1444–1463	132	A or B
	GFEMAR-2	GATAAAGAAGAAACCAGCAG	1556–1575
mecA	GMECAR-1	ACTGCTATCCACCCTCAAAC	1182–1201	163	B
	GMECAR-2	CTGGTGAAGTTGTAATCTGG	1325–1344
eta	GETAR-1	GCAGGTGTTGATTTAGCATT	775–794	93	B
	GETAR-2	AGATGTCCCTATTTTTGCTG	848–867
etb	GETBR-1	ACAAGCAAAAGAATACAGCG	509–528	226	B
	GETBR-2	GTTTTTGGCTGCTTCTCTTG	715–734
tst	GTSSTR-1	ACCCCTGTTCCCTTATCATC	88–107	326	B
	GTSSTR-2	TTTTCAGTATTTGTAACGCC	394–113

^aNucleotide sequence and locations were derived from the published sequences for sea (5), seb (19), sec (7), sed (2), see (11), mecA (23), femA (4), eta (20), etb (16), and tst (6).