TABLE 2.

Predicted sizes of restriction fragments and enzymes used for restriction fragment length polymorphism analysis to validate the amplified products of multiplex PCR

Gene	Amplicon size (bp)	Multiplex primer set	Restriction enzyme used	Expected sizes (bp) of restriction fragments
sea	102	A	AluI	65, 37
seb	164	A	MaeIII	120, 31, 13
sec	451	A	NdeI	307, 144
sed	278	A	MboII	175, 103
see	209	A	AccI	119, 90
femA	132	A or B	AccI	78, 54
eta	93	B	MboI	57, 36
etb	226	B	HpaII	159, 67
tst	326	B	MboI	187, 139
mecA	163	B	MaeIII	84, 79