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Most membrane TRPV4 does not partition into raft domains or interact with caveolar proteins. Higher magnification insets of images Fig. 9C, D. (A) double immunolabeling for TRPV4 and Cav-1; cell nuclei are labeled with DAPI. (B) double immunolabeling for TRPV4 and flotillin; cell nuclei are labeled by DAPI. The scale bar represents 20 μm. DAPI, 4',6-diamidino-2-phenylindole; MβCD, m-β-cyclodextrin; TRPV4, transient receptor potential vanilloid isoform 4.
