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. 2021 Oct 25;62:100145. doi: 10.1016/j.jlr.2021.100145

Fig. 6.

Fig. 6

Membrane cholesterol content regulates stretch dependence of cytoskeletal remodeling. A: TM cells were stretched in the presence/absence of MβCD and MβCD:cholesterol and labeled for filipin (405 nm) and phalloidin-Alexa 488 nm in control, 6% stretch (0.5 Hz, 6%, 1 h), MβCD and MβCD+ stretch samples. B: Averaged and normalized puncta number. Filipin is significantly reduced by stretch and MβCD alone. C: Stretch and MβCD significantly facilitate F-actin fluorescence. Combined stimulation results in an additional ∼30% increase in stress fiber signal (N = 3–4). ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001; ∗∗∗∗P < 0.0001. The scale bar represents 20 μm.