Fig. 3.
Analysis of the sublingual microvasculature and illustration of PBR measurement: (A) Overview on the workflow of performing sublingual microscopy in humans. Parameters such as the perfused boundary region (PBR), an inverse estimate of the eGC thickness, are calculated by the software post-hoc. Image adapted from Rovas et al. [44], modified. (B) Representative image acquired with the GlycoCheckTM system using side-stream darkfield imaging. The software automatically discards invalid segments (yellow lines). Image adapted from Rovas et al. [42], modified. (C) Schematic illustration of the PBR in healthy vascular conditions with intact eGC (low PBR) in contrast to compromised vasculature with damaged eGC (high PBR). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)