figs1.

Immunohistochemical analysis of adropin expression in the mouse brain and levels of adropin in peripheral organs. A, B, Representative confocal microscopy for adropin (red), endothelial cells (CD31, green), and neurons (NeuN, green) as well as nuclei (DAPI, blue) in naïve mouse brain. Adropin and CD31 double labeling show that adropin is highly expressed on endothelial cells in the cerebral cortex (A). Also, adropin is present on NeuN immunoreactive cells in the cortex (B). Scale bars: 10 μm. Data are representative of three independent experiments. C, Representative western blot for adropin in homogenates from different organs of adult (10-12 weeks) naïve male C57/BL6J mice with GAPDH as a loading control. D, E, Densitometric analysis (D), and heatmap (E) show that adropin is highly expressed in endothelial vessel-enriched organs including brain, liver, kidney, and lung, moderately expressed in spleen and intestines, and expressed at very low levels in the heart andskeletal muscle. n=4 per group.