Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 30;4:1342. doi: 10.1038/s42003-021-02845-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a, c, e, g A montage of slices of extracted whole brains of representative 2-mo Mbnl1^+/+/Mbnl2^+/+ (wild-type control) Mbnl1^−/−, Mbnl2^−/−, and Mbnl1^−/−/Mbnl2^+/− mice that underwent in vivo 2D FSE T2w MRIs, respectively, are shown. b, d, f, h Show regions of interest (ROI) manually drawn and color-filled as described in the Methods. Only ROIs that are significantly different from the control Mbnl1^+/+/Mbnl2^+/+ brains are color-filled in the Mbnl1^+/+/Mbnl2^+/+, Mbnl1^−/−, Mbnl2^−/−, and Mbnl1^−/−/Mbnl2^+/− montages. Colored regions are white matter regions, which include the anterior commissure and corpus callosum/external capsule (yellow), and gray matter regions, which include the cerebellum (red), the hippocampus (green), and the hindbrain (pink). Calculated areas from the ROIs were multiplied by the image-slice thickness to generate ROI volumes shown in Figs. 5–7. The olfactory bulb was excluded in volume measurements. Slice positions were identified based on Bregma locations. The slice where the anterior commissure is connected between hemispheres approximates Bregma, which is defined as 0.0 mm. Going from rostral to caudal slice positions, boxes indicate structural landmarks that include Bregma (red box), the last slice containing hippocampal regions (green box), and the end of the brain (yellow box). Slice thickness = 0.5 mm. Scale bar = 1 mm.