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. 2021 Aug 7;219(2):iyab129. doi: 10.1093/genetics/iyab129

Figure 3.

Figure 3

Sup35C restores growth of [PSI+]S cells expressing Sis1JGF. Strain 970TS (sis1Δ expressing Sis1JGF from TRP1 plasmid pYW62 and Sis1 from URA3 plasmid pYW17) was transformed by empty vector (ev) or plasmids encoding Sis1 (pJE240, HIS3), Sup35C (pJE160, HIS3), Sup35MC (pJ528, LEU2), or Sup45 (pJE161, LEU2) together with appropriate empty vectors to maintain all strains on the same selection plate. Transformants were assessed for the ability of Sis1JGF to support growth in place of Sis1 (i.e., on FOA) as in Figure 1. -LHT contains uracil, but lacks leucine, histidine, and tryptophan. YPD and -ade plates show relevant prion phenotypes of transformants. All FOA-resistant strains lack pYW17 and express Sis1JGF from plasmid pYW62. The rightmost column shows cells recovered from FOA, diluted to the same cell density, and grown on rich medium for 2 days to show color and growth phenotypes.