Figure 7.

Sis1JGF phenotypes are consistent across yeast strains and prion variants. Plasmid shuffle as in Figure 1B. The left column shows strains 930, W303, and 74-D694 (all sis1Δ) each with two weak [PSI⁺] variants (W is WSL; 37 is Sc37), two strong variants (STR, S), or no prions (−) indicated above. Each expresses the proteins indicated on the left from a TRP1 plasmid (ev is empty TRP1 vector) and all express wild-type Sis1 from a URA3 plasmid. Hdj-lo is expressed from a single-copy plasmid, while Hdj-hi is overexpressed from a high-copy plasmid. Patches of cells that each contain three pooled transformants were grown on -trp medium containing uracil and limiting adenine (leftmost column) and then replica-plated onto FOA. FOA plates were imaged after incubating 2 days at 30° (2 days) and again after two additional days at 23° (4 days) as indicated. Panels in rightmost column (via Gdn) are from the same -trp lim. ade plates (leftmost column) that were first replica-plated onto YPAD containing 3 mM guanidine and grown overnight. The YPAD plates were then replica-plated onto the FOA plates shown, which were incubated 1 day at 30°. Centers of some patches of 74-D694 cells did not transfer well when replica-plated.