Table 1.
Yeast strains
| Strain | Genotype | Source |
|---|---|---|
| 779-6A | MATa kar1-1 ade2-1 SUQ5 his3Δ202 leu2Δ1 trp1Δ63 ura3-52 | Jung and Masison (2001) |
| W303α | MATα ade1-14 his3-11,15 leu2-3,112 sis1::LEU2 trp1-1 ura3-1/pRS316SIS1 (URA3, SIS1) | Harris et al. (2014) |
| 74-D694a | MATα ade1-14 his3Δ-200 leu2-3,112 trp1-289 ura3-52 sis1::LEU2/pRS316SIS1 (URA3, SIS1) | Harris et al. (2014) |
| 930 | 779-6A MATα sis1::KanMX4/pYW1 7 (URA3, SIS1) [psi–] | Kirkland et al. (2011) |
| 930S | 930, [PSI+]S (endogenous prion) | |
| 970L | 930 + pRS315Sis1JGF (LEU2, sis1JGF)) [psi–] | This study |
| 970LS | 970L, [PSI+]S (endogenous prion) | This study |
| 970LWSL | 970L, [PSI+]WSL (prion donor L1759) | This study |
| 970LSTR | 970L, [PSI+]STR (prion donor 1930 via 779-6A MATa) | This study |
| 970LSc37 | 970L, [PSI+]Sc37 (prion donor 1932 via 779-6A MATa) | This study |
| 970T | 930 + pYW62 (TRP1, sis1JGF) [psi–] | This study |
| 970TS | 970T, [PSI+]S (endogenous prion) | This study |
| 1930 | W303α, [PSI+]STR (endogenous prion) | Harris et al. (2014) |
| 1932 | 1930, [PSI+]Sc37 (endogenous prion) | Harris et al. (2014) |
| 1916 | 1930, [psi–] (cured of prions) | This study |
| 1919 | 1930, [PSI+]WSL (prion donor L1759) | This study |
| 1920 | 1930, [PSI+]S (prion donor 779-6A) | This study |
| 1935 | 74-D694, [PSI+]STR (endogenous prion) | Harris et al. (2014) |
| 1937 | 1935, [PSI+]Sc37 (endogenous prion) | Harris et al. (2014) |
| 1917 | 1935, [psi–] (cured of prions) | This study |
| 1922 | 1935, [PSI+]WSL (1917, prion donor L1759) | This study |
| 1923 | 1935, [PSI+]S (1917, prion donor 779-6A) | This study |
| 1362 | MATa sis1::KanMX SUP35NGMC/pRS314Sis1::FRT, [PSI+]S | This study |
| JS131 | 1362 + pRS313 + pRS315, [PSI+]S | This study |
| JS134 | 1362 + pRS313 + pC5GAL1-FLP, [PSI+]S | This study |
| JS133 | 1362 + pJE240 (HIS3, SIS1) + pRS315, [PSI+]S | This study |
| JS132 | 1362 + pJE240 (HIS3, SIS1) + pC5GAL1-FLP, [PSI+]S | This study |
| JS124 | 1362 + pJE236 (HIS3, sis1JGF) + pRS315, [PSI+]S | This study |
| JS127 | 1362 + pJE236 (HIS3, sis1JGF) + pC5GAL1-FLP, [PSI+]S | This study |
| JS136 | 1362 + pRS313 + pRS315, [psi–] | This study |
| JS135 | 1362 + pRS313 + pC5GAL1-FLP, [psi–] | This study |
| JS139 | 1362 + pJE240 (HIS3, SIS1) + pRS315, [psi–] | This study |
| JS140 | 1362 + pJE240 (HIS3, SIS1) + pC5GAL1-FLP, [psi–] | This study |
| JS138 | 1362 + pJE236 (HIS3, sis1JGF) + pRS315, [psi–] | This study |
| JS137 | 1362 + pJE236 (HIS3, sis1JGF) + pC5GAL1-FLP, [psi–] | This study |
| 628-8Cc | MATa ade2-1 can1 kar1 leu2 lys2 SUQ5 [rho–] (cytoduction tester) | This study |
| MR1118 | 930 + HO::Sup35NM-mKate2 yTRAP sensor | This study |
All strains are [pin–] and all [psi–] strains were obtained by guanidine curing. Strains with designations 1362, 930, 970, and JS are isogenic to 779-6A. The SUP35 allele (NGMC) in strain 1362 has GFP between the N and M domains (Song et al. 2005).
a
74-D694 received from Harris et al. was denoted 74D-694 by them. It is the same 74-D694 first described by Chernoff et al. (1995).