Table 3.
Growth rates (min/cell div)
| Proteins, plasmids | [psi–] | [PSI+]S |
|---|---|---|
| JGF+ev+ev | 155 | na |
| JGF+Sis1+ev | 110 | 117 |
| JGF+Sup35C+ev | 160 | 175 |
| JGF+Sup35MC+ev | 150 | 168 |
| JGF+Sup35C+Sup45 | nd | 149 |
Strain used is 970T (sis1Δ expressing Sis1JGF from a TRP1 plasmid) with combinations of indicated empty vectors (ev) or plasmids encoding indicated proteins (see Figure 3). Cells were grown in YPAD at 30° with constant shaking at 220 rpm. OD600 was measured at 10-min intervals for 24 h. na, not applicable as Sis1JGF [PSI+]S cells are not viable; nd, not determined.