FIG. 1.

sen1-1 and ptk2::TRP1 mutations confer tolerance to different toxic cations and sensitivity to acetic acid. Strain SKY697 (wt) and its derivatives AG149 (ptk2) and AG151 (sen1) were grown in liquid SD medium to saturation, and serial dilutions were dropped on SD plates with either NaCl (0.2 M), LiCl (40 mM), KCl (1.2 M), sorbitol (1.8 M), acetic acid (0.12 M), or tetramethylammonium chloride (TMA, 1 M) or on YPD plates with MnCl₂ (2 mM) or hygromycin B (HygB, 125 μg/ml), as indicated. Growth was recorded after 2 days in the absence of toxic cations and after 3 to 4 days in its presence.