FIG. 3.

sen1-1 mutation is a solo δ-LTR insertion at the PTK2 locus. (A) Scheme of the region of chromosome X between positions 545000 and 548000, containing the PTK2 locus and the site of insertion of a solo δ-LTR. The ORF spans positions 545475 to 547931 on the w-strand, and the 336-bp insertion is at position 545942. sp and asp correspond to the primers used for the PCR in panel B. (B) Products of the PCR performed with chromosomal DNA from strains SKY697 (wt) and AG151 (sen1-1 mutant) with the primers indicated in panel A. Lane M, size markers; their sizes (in base pairs) and that of the amplified bands are indicated at the right and at the left of the figure, respectively.