FIG. 4.

Deletion of the inhibitory domain of Pma1 suppresses the growth phenotypes of the ptk2 mutation. Strains SKY697 (wild type for PTK2, column 1), AG149 (ptk2, column 2), AG205 (ptk2 with plasmid pRS496 expressing truncated Pma1, column 3) and AG224 (ptk2 pma1::URA3-GAL1 promoter-PMA1 with chromosomal ATPase under galactose control and plasmid pRS496 expressing truncated Pma1, column 4) were grown in liquid SD medium to saturation, and serial dilutions were dropped on SD plates with either NaCl (0.2 M) or LiCl (40 mM) as indicated. Growth was recorded after 2 or 4 days in the absence or presence of toxic cations, respectively.