FIG. 5.

Effect of mutations of Pma1 Ser-899 on tolerance to hygromycin B and NaCl of Ptk2⁺ (upper panels) and Ptk2⁻ (lower panels) yeast cells. Wild-type yeast strain W303-1B (PMA1 PTK2) and derivatives with pma1-Ser899 and ptk2 mutations as indicated (strains FPY398, FPY402, FPY1434, FPY1500, and FPY1502) were grown in liquid medium to saturation and diluted 20-fold, and 3 μl was dropped on YPD plates with no addition or containing hygromycin B (50 μg/ml) or NaCl (1.2 M), as indicated. Growth was recorded after 4 days.