TABLE 2.
Effect of the sen1-1 mutation on the uptake of lithium and methylammoniuma
| Strain | Avg cation uptake (nmol/mg of cells) ± SD
|
|
|---|---|---|
| Lithium | Methylammonium | |
| SKY6697 (ena1-4 SEN1) | 27.2 ± 1.3 | 26.2 ± 0.2 |
| AG151 (ena1-4 sen1-1) | 17.3 ± 0.8 | 22.3 ± 0.4 |
a
Results are the average (± standard deviation) of three determinations, and the uptake experiments were repeated twice with similar (± 10%) results. The external concentrations of lithium and methylammonium were 40 and 0.25 mM, respectively. Uptake times were 90 min, corresponding to the steady-state level of intracellular cation (33).