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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Sci Total Environ. 2021 Sep 30;806(Pt 2):150703. doi: 10.1016/j.scitotenv.2021.150703

Fig. 5. Detection of DNA damage marker (γH2AX) and oxidative DNA damage (8-OHdG) in the liver.

Fig. 5.

(A) Representative Western blotting images of H2AX and γH2AX using liver whole-cell lysates. (B) Protein levels were quantified by densitometry analysis and normalized to total protein loading per sample (N = 6/group). Relative abundance was reported as fold of control (WT-CON). Results are presented in Scatter plots showing individual data points from each experimental group with the group median at the line (N = 6/group). Differences between groups were analyzed using two-way ANOVA with post-hoc Bonferroni test correction for the main effects of DX exposures (PT), genotypes (PG), or the interaction between these two factors (PI). P < 0.05 was considered significant. (C) IHC detection of γH2AX in liver tissues. Magnifications, 100x (I) and 400x (II). (D) Liver 8-OHdG Levels were measured by a competitive ELISA assay. Results are reported as pg/μg genomic DNA and presented in Box plots showing individual data points from each experimental group (N = 6/group). Group differences were analyzed using one-way ANOVA with post-hoc Dunnett’s test correction. *P < 0.05 was considered significant. CON, provided regular water; DX-3m, exposed to DX in drinking water for 3 months.