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. 2021 Nov 30;11:23181. doi: 10.1038/s41598-021-02388-8

Figure 7.

Figure 7

Sample 7. Image of a wet ulcer of 12 weeks duration in the lower limb (a); Fluorescence in situ hybridization showing bacteria in red with the CY3 tagged Eu bacterial probe, extracellular polymeric substances in green with Concavalin A conjugated Alexa Fluor 488 and tissue nuclei in blue with DAPI (b); Gram staining showing gram positive cocci (c); Scanning electron microscopy showing coccoid shaped bacteria in chains embedded in smooth extracellular polymeric substance (d).