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. 2021 Nov 30;11:23181. doi: 10.1038/s41598-021-02388-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sample 27. Image of a wet ulcer of 8 weeks duration in the lower limb with green colour pus discharge (a); Fluorescence in situ hybridization showing bacteria in red with the CY3 tagged Eu bacterial probe, extracellular polymeric substances in green with Concavalin A conjugated Alexa Fluor 488 and tissue nuclei in blue with DAPI (b); Gram staining showing gram-negative rods (c); Scanning electron microscopy showing bacteria in embedded in smooth extracellular polymeric substance (d).