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. 2021 Dec 1;10:38. doi: 10.1186/s13619-021-00099-z

Fig. 1.

Fig. 1

Stage-wise Immunohistochemistry of the cells undergoing differentiation. HiPSC stained for OCT4, NANOG, PAX6 and SOX17 showed expression of pluripotency markers only. Definitive endoderm show staining for PAX6, SOX17 and GATA4,and other oancretaic markers, Pluripotency markers remain negative