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. 2021 Mar 2;42(12):2058–2068. doi: 10.1038/s41401-021-00618-3

Fig. 7. Roxithromycin downregulated the expression of NOX4 in lung fibrosis.

Fig. 7

a The mRNA level of NOX4 in the lungs of the mice from the indicated groups was evaluated with qPCR analysis. b The mRNA level of NOX4 in the MRC-5 cells stimulated with TGF-β or RXM was determined by qPCR analysis. c–f Twenty-four-well-cultured MRC-5 and A549 cells were transfected with 1 μL of siNOX4 for 24 h. MRC-5 cells were then treated with 5 ng/mL TGF-β alone or with 40 μM RXM for another 24 h. A549 cells were treated with 15 μg/mL BLM alone or with 40 μM RXM for another 3 days. The cells were harvested for further analysis. c, d The knockdown efficiency of NOX4 in MRC-5 and A549 cells was confirmed by qPCR and Western blot analysis, respectively. e Detection of fibrotic markers in MRC-5 cells was performed by Western blot analysis. f Evaluation of MMP-9 in A549 cells was performed with qPCR analysis. The results are presented as the mean ± SEM from three independent experiments. Statistical significance, *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001.