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. 2021 Nov 30;11:23176. doi: 10.1038/s41598-021-02638-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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LDA effect size analysis showing differentially abundant taxa between patients with LNM and non-LNM with a threshold of |log10 LDA|≥ 2. Bar chart showing Log10 LDA of differential taxa in OSCC patients between metastasis and no metastasis groups (A). Cladogram showing the hierarchy among discriminative taxa. From phylum to genus, each node represents a taxonomic level. Red nodes demonstrate enriched taxa in the no metastasis group; green nodes represent enriched taxa in the metastasis group, LDA; linear discriminant analysis, OSCC; oral squamous cell carcinoma.