Skip to main content
. 2021 Nov 30;12:6984. doi: 10.1038/s41467-021-27306-4

Fig. 4. SIPAN are a general phenomenon common to many mammalian cell types.

Fig. 4

a, b Immunostaining of endogenous PSMD4 or PSMD7 in diverse cell types, showing that these proteins localize in nuclear foci following incubation in HBSS. SIPAN are observed in normal primary cells (e.g., HDLF: primary human lung fibroblasts, HUVEC: human endothelial cells, mESC: mouse embryonic stem cell); immortalized cells (e.g., NIH3T3: mouse embryonic fibroblasts, C2C12: mouse myoblast cell line, 3T3L1: mouse preadipocytes); transformed (e.g., Cos7: Transformed monkey kidney cells, RAW264.7: Abelson murine leukemia virus transformed macrophage) and tumoral cells (MCF7: human breast cancer, H1299: human non-small cell lung cancer). c Immunostaining of PSMD7 in mouse pre-adipocytes 3T3L1 and differentiated adipocytes showing that this protein localizes in nuclear foci following incubation in HBSS. Right panel, Oil Red O staining was conducted to control for adipocyte differentiation (representative images from 3 independent experiments).