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. 2021 Mar 3;42(12):2106–2119. doi: 10.1038/s41401-021-00617-4

Fig. 1. HIF-1α activation is associated with poor AKI outcomes.

Fig. 1

a Serum creatinine (Scr) levels on day 14 after 30 or 40 min ischemia/reperfusion-induced renal injury (I/RI). b Representative histological changes (periodic acid-Schiff [PAS] staining) on day 14 after 30 or 40 min of I/RI (n = 7). Scale bar, 100 μm. c Representative images of Masson staining (n = 7). Scale bars, 100 μm. d, e Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting analyses of α-SMA, collagen-1, and fibronectin expression. The relative levels were normalized to β-actin or GAPDH (n = 7). f Representative images of α-SMA and collagen-1 immunostaining on day 14 in kidneys from mice subjected to 30 or 40 min I/RI (n = 7). Scale bar, 100 μm. g Western blotting analyses of HIF-1α expression in kidney tissues (n = 7). h Representative images of HIF-1α expression in kidney tissues from mice after 30 or 40 min of I/RI. Scale bar, 50 μm. Data are shown as the mean ± SEM of 7 mice. **P < 0.01 versus the sham control (Dunnett’s test). GAPDH, glyceraldehyde-3-phosphate dehydrogenase.