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. 2021 Nov 30;6:83. doi: 10.1038/s41536-021-00193-5

Fig. 6. Alteration of myofibroblasts morphology and specific markers in the presence of IL-10 and co-culture with M2 macrophages subtypes.

Fig. 6

a Representative images showing nuclei (blue), actin filaments (gray), and αSMA (green) (scale bar: 50 μm). Myofibroblast de-dedifferentiation were analyzed using b percentage of αSMA-positive cells by manual counting of cells with αSMA incorporation into actin stress fibers and quantitative analysis of c αSMA, d Coll1a1, and e EDA-FN gene expression. For quantitative analysis of αSMA-positive cells, at least 200 cells per condition were counted. Data are represented as mean ± SD; *significance level of p ≤ 0.05 using a one-way ANOVA followed by Tukey’s post hoc analysis). The characters # and § represent the significance level of p ≤ 0.05 using a one-way ANOVA followed by Tukey’s post hoc analysis when compared to control, and treated samples with IL-10, respectively. Experiments were performed in four replicates.