TABLE 1.
Selected algae species and their growth conditions.
Chloromonas brevispina | Kremastochrysopsis austriaca | Dunaliella salina | Spirulina plantensis | Chlorella vulgaris | |
Classification | Snow (Psychrophilic) | Snow (Psychrophilic) | Halophilic | Mesophilic | Mesophilic |
Media | M1b | M1b | 2X Erd Mediumc | Enriched Seawater Mediumd | Proteose Mediume |
Pressures (mbar)f | (OD750, cell cts)g 670 ± 20, 330 ± 20, 160 ± 20, 80 ± 2.5 | (OD750)g 670 ± 20, 330 ± 20, 160 ± 20, 80 ± 2.5 | (OD750, cell cts)g 670 ± 20, 330 ± 20, 160 ± 20, 80 ± 2.5 | (OD750)g 670 ± 20, 330 ± 20 | (OD750, cell cts)g 670 ± 20, 330 ± 20, 160 ± 20, 80 ± 2.5 |
Temperatures (°C) | 4.0 ± 0.1 | 4.0 ± 0.1 | 20.8 ± 2.6 (10.0 ± 0.1 at 80 ± 2.5 mbar) | 20.8 ± 2.6 | 20.8 ± 2.6 (10.0 ± 0.1 at 80 ± 2.5 mbar) |
Light levels (aμmol m–2s–1) | 62–70a | 62–70a | 62–70a | 62–70a | 62–70a |
Incubation time | 33–54 days | 33–54 days | 33–62 days | 62 days | 33–62 days |
aAs measured using a handheld digital lux meter (URCERI) ± 3% accuracy, where the 62–70 μmol of photons m–2 s–1 range was chosen based on previous work by Harrold et al. (2018).
bM1 medium (Hoham et al., 1979).
c2X Erdschreiber’s Medium (modified Erdschreiber’s medium, Foyn, 1934).
dEnriched Seawater Medium (Lewin, 1979).
eProteose Medium (Bold, 1949).
fValues of uncertainties are based on the accuracy of the gauges monitoring the pressure.
gBoth cell counts (cell cts) and OD750 readings were measured for C. brevispina, D. salina and C. vulgaris and for K. austriaca and S. plantensis only OD750 readings were measured due to their minimal growth.