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. 2021 Sep 3;19(12):2606–2618. doi: 10.1111/pbi.13686

Figure 2.

Figure 2

Generation and identification of the pOsl2::GWD1‐overexpressing transgenic rice plants and gwd1 knock‐down mutants. (a) Schematic diagram showing the structure of the pOsl2::GWD1 transformation construct. Osl2 pro, Osl2 promoter; 35S term, CaMV 35S terminator. (b) PCR analysis of total DNA from representative pOsl2::GWD1 transgenic rice lines and the WT. (c) Analysis of the potential cis‐elements in the GWD1 core promoter. (d) DNA sequences around the editing site and identification of gwd1 mutants. The guide RNA sequence used in CRISPR/Cas9‐mediated gene editing of GWD1 is highlighted in red, and the protospacer adjacent motif (PAM) is highlighted in blue. The deletion in gwd1‐1 is indicated by a hyphen. Expression analysis of GWD1 in the leaves of pOsl2::GWD1 overexpression lines (e) and gwd1 mutants (f). Error bars represent the SDs. Each experiment was repeated at least three times. **P < 0.01 (Student’s t‐test)