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. 2021 Sep 16;19(12):2415–2427. doi: 10.1111/pbi.13649

Figure 4.

Figure 4

Functional depiction of role of CaRR13 during root nodulation in chickpea. (a) Overexpression of CaRR13 in chickpea through hairy root transformation. (b) Knockdown analysis using RNAi of CaRR13 in chickpea through hairy root transformation. (c) Relative quantification of CaRR13 and NSP2 expression in overexpression lines compared to respective vector controls. (d) Relative quantification of CaRR13 and NSP2 expression in RNAi lines compared to respective vector controls. (e) The expression profiling of TypeB RRs in CaRR13 RNAi knockdown lines. (f) Change in nodule number upon overexpression and RNAi lines of CaRR13 in comparison to respective vector control (* represents t‐test P‐value ≤ 0.05). (g) Complementation of ∆cre1‐1 and ∆cre1‐2 Medicago mutants with CaRR13 (h) Bar diagram represents the number of nodules in the CaRR13 complemented ∆cre1‐1 mutant lines (*** represents t‐test P‐value ≤ 0.0001). (i) Bar diagram represents the number of nodules in the CaRR13 complemented ∆cre1‐2 mutant lines (*** represents t‐test P‐value ≤ 0.0001). The experiments were carried out in triplicates (n = 15 plants) and expressed as means ± SE.