. 2021 Jul 28;19(12):2442–2453. doi: 10.1111/pbi.13670

Table 2.

Terpene and phenolic composition of chicory leaves of WT and CiGAS chicory lines

Line	CiGAS gene genotype				Sesquiterpene lactone and phenolic compound quantification
Line	S1	S2	S3	L	L µg/gFW	LP µg/gFW	8‐DOL relative abundance	Lox relative abundance	LPox relative abundance	8‐DOLox relative abundance	CA µg/gFW	CiA µg/gFW	3,5‐DiCQA µg/gFW
PL1	W/W	W/W	W/W	W/W	6.8 ± 2.1^ab	10.7 ± 3.2^abc	10.2 ± 2.8^bc	149.6 ± 55.4^c	320.2 ± 120.2^b	154.1 ± 50.4^e	95.0 ± 26.6 ^a	1535.6 ± 55.7^ab	172.8 ± 9.9^a
PL2	W/W	W/W	W/W	W/W	4.3 ± 0.0^ab	8.0 ± 0.5^abc	9.2 ± 0.1^abc	104.5 ± 7.6^c	259.2 ± 6.4^b	140.7 ± 9.9^de	131.7 ± 4.9 ^a	1649.3 ± 58.0 ^ab	227.9 ± 13.1^a
PL3	W/W	W/W	W/W	W/W	5.9 ± 1.6^ab	12.4 ± 4.7^bc	9.2 ± 2.4^abc	118.3 ± 15.8^c	317.7 ± 65.4^b	126.8 ± 13.2^de	74.7 ± 4.3 ^a	1608.1 ± 18.4^ab	164.0 ± 31.5^a
PL4	W/W	W/W	W/W	W/W	9.9 ± 4.4^b	15.9 ± 7.5^c	19.4 ± 8.6^c	127.1 ± 26.3^c	293.8 ± 78.5^b	166.4 ± 37.6^e	138.7 ± 33.9 ^a	1963.3 ± 152.8^ab	172.2 ± 58.8^a
RN1	W/W	W/W	W/W	W/W	4.1 ± 0.7^ab	6.2 ± 0.9^abc	8.0 ± 1.8^ab	88.7 ± 14.4^bc	195.4 ± 24.9^b	112.6 ± 27.3^de	56.4 ± 23.9 ^a	1320.3 ± 195.8^ab	122.5 ± 5.9^a
RN2	W/W	W/W	W/W	W/W	4.7 ± 2.8^ab	7.2 ± 3.0^abc	7.3 ± 3.8^ab	117.5 ± 11.4^c	250.7 ± 0.2^b	127.6 ± 3.0^de	92.6 ± 9.7 ^a	1531.1 ± 163.9^ab	152.4 ± 1.8^a
RN3	W/W	W/W	W/W	W/W	3.0 ± 0.2^ab	3.8 ± 0.8^ab	3.0 ± 0.5^ab	120.2 ± 32.5^c	200.0 ± 77.3^b	79.9 ± 33.9 cd	72.5 ± 5.7 ^a	1417.1 ± 382.2^ab	109.1 ± 11.4^a
RN4	−5/−11	−9/−9	−7/ins	W/W	0.2 ± 0.1^a	0.6 ± 0.5^a	0.3 ± 0.3^ab	0.1 ± 0.1^a	8.3 ± 7.5^a	1.2 ± 1.2^a	52.0 ± 3.7 ^a	1360.2 ± 237.1^ab	164.8 ± 5.4^a
RN5	−12/−9	W/W	W/W	W/W	6.7 ± 4.5^ab	8.2 ± 4.2^abc	5.5 ± 3.3^ab	120.1 ± 12.1^c	221.4 ± 2.9^b	77.0 ± 6.0^bcd	109.6 ± 1.3 ^a	1437.5 ± 100.5^ab	166.0 ± 31.2 ^a
RN6	−19/−4	W/W	−4/W	W/W	6.1 ± 2.8^ab	11.1 ± 4.8^abc	8.7 ± 4.5^ab	114.7 ± 3.6^c	289.2 ± 5.8^b	115.4 ± 6.1^de	102.7 ± 13.2 ^a	1773.4 ± 215.1^ab	227.6 ± 7.1^a
RN7	−4/−17	−11/−11	−2/−7	W/W	0.3 ± 0.1^a	0.7 ± 0.1^a	0.7 ± 0.2^ab	0.0 ± 0.0^a	16.3 ± 2.0^a	4.6 ± 0.9^a	92.6 ± 38.8 ^a	1404.9 ± 157.7^ab	151.6 ± 51.4^a
RN8	−14/−7	−13/−10	−2/W	W/W	2.0 ± 0.1^a	8.4 ± 1.0^abc	4.8 ± 1.2^ab	35.2 ± 5.1^ab	267.5 ± 0.3^b	79.8 ± 7.6 cd	63.6 ± 7.2 ^a	1720.2 ± 151.5^ab	156.3 ± 7.4^a
RN9	−9/−9	−10/W	−4/−4	W/W	4.2 ± 2.2^ab	5.6 ± 2.6^abc	5.2 ± 2.4^ab	88.8 ± 15.1^bc	166.9 ± 30.3^ab	70.3 ± 18.1^abcd	100.4 ± 12.3 ^a	1133.6 ± 198.0^ab	104.9 ± 12.2^a
RN10	−3/−5	−6/−3	−7/W	W/W	0.2 ± 0.0^a	0.4 ± 0.1^a	0.3 ± 0.1^ab	0.0 ± 0.0^a	7.0 ± 1.8^a	1.3 ± 0.4^a	124.0 ± 56.6 ^a	1615.3 ± 564.9^ab	222.3 ± 1.6^a
RN11	−7/−7	−5/−5	−2/−2	W/W	0.2 ± 0.0^a	0.1 ± 0.0^a	0.1 ± 0.1^ab	0.0 ± 0.0^a	0.4 ± 0.5^a	0.1 ± 0.1^a	96.5 ± 78.7 ^a	967.9 ± 690.1^a	156.1 ± 119.7^a
RN12	−4/−7	+1/−7	−7/−16	W/W	0.3 ± 0.0^a	0.9 ± 0.2^a	0.6 ± 0.3^ab	0.0 ± 0.0^a	21.4 ± 10.4^a	4.3 ± 3.2^a	68.6 ± 23.4 ^a	1428.9 ± 33.0^ab	201.2 ± 4.4^a
RN13	−11/−11	−12/−6	−9/−7	W/W	0.3 ± 0.0^a	0.5 ± 0.1^a	0.7 ± 0.3^ab	0.0 ± 0.0^a	9.2 ± 3.0^a	2.7 ± 0.8^a	142.6 ± 4.4 ^a	1812.5 ± 350.9^ab	283.0 ± 3.5^a
RN14	−7/−7	−9/−7	−3/−3	−11/W	0.4 ± 0.2^a	0.7 ± 0.4^a	0.6 ± 0.4^ab	0.0 ± 0.0^a	9.5 ± 6.3^a	2.5 ± 2.3^a	89.7 ± 12.1 ^a	1928.7 ± 386.8^ab	192.8 ± 7.0^a
RN15	W/−16	W/W	W/−5	W/W	4.4 ± 1.2^ab	7.6 ± 3.9^abc	7.4 ± 2.1^ab	89.6 ± 0.8^b	191.6 ± 30.2^b	107.3 ± 18.1^de	94.8 ± 39.8 ^a	1266.8 ± 250.1^ab	116.5 ± 35.3^a
PL5	−9/−12	W/W	−9/−2	−6/−6	5.8 ± 1.4^ab	9.2 ± 4.4^abc	9.3 ± 1.3^abc	99.1 ± 19.8^b	214.2 ± 82.0^b	101.7 ± 17.4^de	71.3 ± 16.2^a	1479.7 ± 113.9^ab	128.4 ± 43.2^a
RN16	−2/−11	−2/−11	−9/−7	−7/−16	0.5 ± 0.4^a	0.7 ± 0.4^a	0.7 ± 0.5^ab	0.0 ± 0.0^a	16.9 ± 19.8^a	7.6 ± 9.4^ab	176.0 ± 119.5^a	2608.0 ± 1100.1^b	426.6 ± 473.0^a
RN17	−6/−5	−10/−6	−11/+1	−8/−9	0.1 ± 0.0^a	0.1 ± 0.0^a	0.0 ± 0.0^a	0.0 ± 0.1^a	0.3 ± 0.2^a	0.0 ± 0.0^a	107.8 ± 59.0^a	1009.9 ± 158.6^a	61.6 ± 56.1^a
PL6	−1/−1	−4/−4	−2/−11	−1/−5	0.4 ± 0.2^a	0.7 ± 0.5^a	0.9 ± 0.5^ab	0.0 ± 0.0^a	17.3 ± 17.5^a	8.9 ± 8.2^abc	98.2 ± 29.5^a	1747.7 ± 168.8^ab	296.8 ± 90.8^a
PL7	−3/−3	−2/−2	−4/−4	−5/−5	0.6 ± 0.1^a	1.1 ± 0.2^a	1.1 ± 0.1^ab	0.0 ± 0.0^a	22.2 ± 13.6^a	7.4 ± 4.7^ab	97.9 ± 59.9^a	1992.1 ± 547.0^ab	228.2 ± 23.4^a

The mean ± SD is shown for duplicate measurements. The letters indicate significantly different groups according to ANOVA and the Tukey’s post hoc test (P < 0.05). CA, chlorogenic acid; CiA, chicoric acid; 3,5‐DiCQA, 3,5‐dicaffeoylquinic acid; 8‐DOL, 8‐deoxylactucin; 8‐DOLox, 8‐deoxylactucin oxalate; ins, large insertion; L, lactucin; Lox, lactucin oxalate; LP, lactucopicrin; LPox, lactucopicrin oxalate; W, wild‐type genotype.