Skip to main content
. 2021 Aug 25;19(12):2501–2516. doi: 10.1111/pbi.13676

Figure 4.

Figure 4

Seasonal flowering and the analysis of floral organ identity and flowering‐time‐related genes. (a) Schematic diagram of C. sinense seasonal growth and development in 12‐month cycle. (b) Floral development of C. sinense. (a–c) Scanning electron micrograph (SEM) of early floral developmental stages. (a) Dormant lateral buds (S0). (b) potential floral bud initiation in the lateral buds of developing shoots (S1). (c) Developing floret. d‐f. developing flowers, Bar = 1 cm. The developing flower of stage 3 (d), stage 4 (e) and mature flowers (f). The first line: plant morphology, the second line: flower bud or floret form, the third line: microstructure of floral bud and floral organ. Im, inflorescence meristem; Br, Bract; FP, floret primordium; SP, sepal primordium; PP, petal primordium; LP, labellum primordium; CP, column primordium. Se, sepal, Pe, petal, Li, lip and Co, column, AC, anther cap, Lo, locule. Po, pollinium. (c) Biomolecular fluorescence complementation visualization. Fusion proteins CsSVP3‐YFPn and CsAP1‐YFPc or CsSOC1‐YFPc were co‐expressed in C. sinense protoplasts and YFP signals were detected in nuclei where the DAPI signal presented, while negative controls did not produce any BiFC fluorescence. (d) Control of flowering in C. sinense. Arrows with solid line indicated positive interaction, and right angle indicated negative interaction.