Figure 6.

Evolution and expression of key genes involved in anthocyanin biosynthesis in Cymbidium sinense. (a) The differential expression patterns of genes involved in anthocyanin biosynthesis in four different colour flowers of C. sinense. (b) The phylogenetic tree of 2ODD genes among Cymbidium sinense, Epimedium sagittatum, Albuca bracteata, Dioscorea alata, Dendrobium officinale, Citrus clementine, Camptotheca acuminate, Actinidia rufa, Curcuma alismatifolia, Strelitzia reginae, Apostasia shenzhenica, Phalaenopsis equestri, Setaria italic, Oryza sativa, Zea mays, Vigna radiate, Dendrobium catenatum, Gymnadenia rhellican, Dendrobium hybrid, Doritaenopsis hybrid, Cymbidium hybrid, Arabidopsis thaliana, Oncidium hybrid, Indosasa hispida, Triadica sebifera, Lotus sessilifolius, Lotus filicaulis, Vitis vinifera, Gypsophila elegans, Glycine max, Lagerstroemia indica, Mucuna pruriens, Arachis hypogaea, Paeonia lactiflora, Canarium album, Litchi chinensis, Gossypium barbadense, Hibiscus sabdariffa, Prunus cerasifera, Dimocarpus longan, Glycine soja, Rosa rugosa, Malus domestica, Sorghum bicolor, Osmanthus fragrans, Colobanthus quitensis, Apocynum venetumv, Salvia splendens, Crocus sativus, Allium cepa, Vaccinium corymbosum, Camellia sinensis, Nicotiana tabacum, Nicotiana tomentosiformis, Nicotiana sylvestris. (c) The phylogenetic tree and multiple alignment analysis of F3'5'H from Cymbidium sinense (highlighted in red) and other members of Orchidaceae. Positions 5, 8 and 10 of CR1 and SRS6 amino acids are shown in black squares. (d) The phylogenetic tree of MYB genes among Cymbidium sinense, Apostasia shenzhenica, Oryza sativa, Zea mays, Petunia hybrida, Dendrobium spp, Capsicum annuum, Solanum lycopersicum, Antirrhinum majus, Oncidium spp. Gower Ramsey, Malus domestica, Ipomoea batatas, Vitis vinifera, Phalaenopsis equestris, Phalaenopsis spp and gene expression patterns of MYB genes in four different colour flowers of Cymbidium sinense.