Fig. 4.
Validations for PDH-dependent apoptotic cell death using PDHA1-manipulated A549 cells. PDHA1WT (WT) and PDHA13SD (3SD) A549 cells were treated with DCA (50 mM; A), JX06 (50 μM; B), AZD7545 (20 μM; C), VER-246608 (50 μM; D), Leelamine (10 μM; E), huzhangoside A (0.1 μM; F) and otobaphenol (10 μM; G) for 24 h. The cells stained with Annexin Ⅴ and propidium iodide (PI) were analyzed by flow cytometry. (H) The percent frequency of Annexin Ⅴ-positive apoptotic cells is shown as means ± SEM. **P < 0.01; ***P < 0.001.