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Effect of human serum components on the detection of NS1 in the capture ELISA. Immunoaffinity-purified, secreted NS1 was serially diluted in the absence (●) or presence of NHS at dilutions of 1 in 5 (Δ) and 1 in 10 (○) and probed with the type-specific MAb 1H7.4. Data points represent the mean ± standard deviation for four replicates. The dashed line is the mean for the negative control samples (OD490, 0.122 ± 0.012).
