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. 2021 Nov 2;297(6):101379. doi: 10.1016/j.jbc.2021.101379

Figure 1.

Figure 1

CASP6 contributes to CASP11-NLRP3 inflammasome activation. A, the immunoblot analysis of pro- (p45) and cleaved caspase-1 (p20; CASP1) in bone marrow-derived macrophages (BMDMs) after Escherichia coli infection at an MOI of 20 for 20 h. B and C, IL-1β (B) and IL-18 (C) release from BMDMs infected with E. coli at an MOI of 20 for 20 h. D, immunoblot analysis of pro- and cleaved CASP1 in BMDMs after Citrobacter rodentium infection at an MOI of 20 for 20 h. E and F, IL-1β (E) and IL-18 (F) release from BMDMs infected with C. rodentium at an MOI of 20 for 20 h. G, immunoblot analysis of pro- and cleaved CASP1 in BMDMs after Pseudomonas aeruginosa mutant strain ΔpopB infection at an MOI of 20 for 20 h. H and I, IL-1β (H) and IL-18 (I) release from BMDMs infected with ΔpopB at an MOI of 20 for 20 h. The data are representative of at least three independent experiments. The data are shown as mean ± SEM (B, C, E, F, H, and I). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001 (one-way ANOVA). CASP, caspase; MOI, multiplicity of infection; NLRP, nucleotide-binding oligomerization domain-like receptor containing pyrin domain.