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. 2021 Nov 12;8(6):rbab061. doi: 10.1093/rb/rbab061

Table 1.

Primer sequences used in quantitative real-time PCR

Gene Primers
GAPDH (rat) Forward: 5′-CGGCAAGTTCAACGGCACAGTCAAGG-3′
Reverse: 5′-ACGACATACTCAGCACCAGCATCACC-3′
ALP (rat) Forward: 5′-ACAAGGTGGTGGACGGTGAAC-3′
Reverse: 5′-CGTGAAGCAGGTGAGCCATAGG-3′
RUNX2 (rat) Forward: 5′-ACCAGCAGCACTCCATATCTCTAC-3′
Reverse: 5′- CTTCCATCAGCGTCAACACCAT-3′
Col-1α1 (rat) Forward: 5′-CAACAGACTGGCAACCTCAAGAAG-3′
Reverse: 5′-CACAAGCGTGCTGTAGGTGAATC-3′
VEGFA (rat) Forward: 5′-CGTCCTGTGTGCCCCTAAT-3′
Reverse: 5′-TGGCTTTGGTGAGGTTTGAT-3′
ANGPT-1 (rat) Forward: 5′-TATGGATGTGAATGAAGGAGGATGG-3′
Reverse: 5′-ACTGCCTCTGACTGGTTATTGC-3′
FGF-2 (rat) Forward: 5′-CTGGCTATGAAGGAAGATGGAC-3′
Reverse: 5′-CGGTAAGTGTTGTAGTTATTGGAC-3′
GAPDH (human) Forward: 5′-ACCCACTCCTCCACCTTTGAC-3′
Reverse: 5′-TCCACCACCCTGTTGCTGTAG-3′
HIF-1α (human) Forward: 5′-AGGACACAGATTTAGACTTGGAGATG-3′
Reverse: 5′-CAGTGGTAGTGGTGGCATTAGC-3′
eNOS(human) Forward: 5′-TTGTCTGCGGCGATGTTACC-3′
Reverse: 5′-GCGTATGCGGCTTGTCACC-3′
VEGFA (human) Forward: 5′-CGCTTACTCTCACCTGCTTCTG-3′
Reverse: 5′-TCCAACAATGTGTCTCTTCTCTTCG-3′
KDR (human) Forward: 5′-CGCAGAGTGAGGAAGGAGGAC-3′
Reverse: 5′-CCGTAGGATGATGACAAGAAGTAGC-3′
TEK (human) Forward: 5′-GCAGAGAACAACATAGGGTCAAGC-3′
Reverse: 5′-AGGTCATTCCAGCAGAGCCAAG-3′
FGFR1 (human) Forward: 5′-CTGGGAGAGGGCTGCTTTGG-3′
Reverse: 5′-CACTTTGGTCACACGGTTGGG-3′