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. 2021 Feb 2;33(4):1161–1181. doi: 10.1093/plcell/koab024

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�The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transformation and localization of a subset of recombineered targets. A, Chlamydomonas transformation pipeline. The I-SceI cut site allows vector linearization prior to Chlamydomonas transformation via electroporation. Transformants are directly screened for fluorescence using a Typhoon scanner (GE Healthcare, San Diego, CA, USA) and then picked and propagated prior to imaging. GOI: gene of interest. B, The localizations of a subset of the recombineered target genes. Localizations agree with data from an affinity-purification followed by mass spectrometry study (Mackinder et�al., 2017) or pyrenoid proteomics (Zhan et�al., 2018 and/or Mackinder et�al., 2016). Scale bars: 5 �m.