protein | antibody | concentration used | species |
---|---|---|---|
PP1 | Sc-7482 | 1 : 1000 | mouse |
total eIF2α | Ab26197 | 1 : 1500 | rabbit |
P-eIF2α | Ab32157 | 1 : 1000 | rabbit |
vinculin | 4650S | 1 : 5000 | rabbit |
FLAG | F7425 | 1 : 1000 | rabbit |
actin | 20536-1-AP | 1 : 10 000 | rabbit |
anti-rabbit IgG (H + L), HRP conjugate | W4011 | 1 : 10 000 | goat |
anti-mouse IgG (H + L), HRP conjugate | W4021 | 1 : 10 000 | goat |