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. 2021 Nov 12;12:755961. doi: 10.3389/fimmu.2021.755961

Figure 3.

Figure 3

See also Supplementary Figure S3 Caspase-11 synergizes with MT3 in impairing bacterial clearance. WT, Casp-11-/- , Mt3-/- and Casp-11-/-Mt3-/- mice were infected i.p. with E. coli (1 X109 CFUs/mouse) for 6h. (A) Bacterial CFUs measured in kidney, blood and peritoneal lavage, n = 3-6 per group, one-way ANOVA. (B) Western blots of pro-GSDMD, active-GSDMD (p31), pro-caspase-1, active-caspase-1, pro-IL1β and active-IL-1β in kidney homogenates, n = 3-6 per group, one-way ANOVA, data are mean ± SEM. (C) WT and Mt3-/- mice treated i.p. with MCC950 (1 mg/mouse) or PBS and infected i.p. with E. coli (1 X109 CFUs/mouse) for 6h. IL1β was measured by ELISA in peritoneal lavage, n = 6 per group, one-way ANOVA, data are mean ± SEM. Bacterial CFUs in whole blood and kidney, n = 4 per group, one-way ANOVA, data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.