Skip to main content
. 2021 Oct 29;1:44. doi: 10.1038/s43856-021-00044-w

Fig. 3. Differentiation of hiPSCs into OPCs and SCPs and confirmation of SARS-CoV-2 cell entry-related protein expression in these cell types.

Fig. 3

a, b Scheme illustrating the generation of OPCs and SCPs from hiPSC and a bright-field view of them. Scale bars = 50 μm. c Expression of ACE2, TMPRSS2, and FURIN mRNA in OPCs and SCPs differentiated from hiPSC line UCSD112i-2-11 relative to hiPSCs. Individual gene expression levels in hiPSCs are set to 1. N = 3 biological replicates, 3 technical replicates; n.s. > 0.05, *P < 0.05, **P < 0.01, ****P < 0.0001; mean ± SEM. d Expression of SARS-CoV-2 entry proteins together with pluripotency markers (SSEA4, OCT4, NANOG) in from hiPSC line UCSD112i-2-11, otic markers (PAX2, PAX8, COCH) in OPCs, and Schwann cell precursor markers (GAP43, SOX10, CDH19) in SCPs. Data are representative images from at least 3 independent differentiations. Scale bars = 50 μm.