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. 2021 Oct 29;1:44. doi: 10.1038/s43856-021-00044-w

Fig. 5. Hair cell-like cells and neurons from inner ear organoids infected with SARS-CoV-2.

Fig. 5

a Schematic overview of the generation of inner ear organoid from hiPSCs and infection with SARS-CoV-2. b–d Protein expression of ACE2 in inner ear vestibular organoids containing MYO7A+ hair cells (b), TUBB3+ cell body of ganglion neurons (c), and MBP+ Schwann cells (d) generated from hiPSC line SK8-A. Scale bars = 10 μm. e–h Immunofluorescent staining of mock (e) and SARS-CoV-2-infected differentiated organoid culture. Viral dsRNA, stained by the J2 antibody, colocalized with the hair cell marker MYO7A (shown in (f) and (g)) and neuronal marker MAP2 (shown in (h)). Scale bars = 20 μm. Representative images from at least 3 independent organoids.