REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD4-PerCP-Cy5.5 (RM4-5)	eBioscience	Cat#45-0042-82;RRID:AB_1107001
CD4-FITC (GK1.5)	Biolegend	Cat#100406; RRID:AB_312691
CD4-PE (GK1.5)	Biolegend	Cat#100408; RRID:AB_312693
CD45R/B220-APC (RA3-6B2)	Biolegend	Cat#103212; RRID:AB_312997
CD45R/B220-PE (RA3-6B2)	eBioscience	Cat#12-0452-82; RRID:AB_465671
CD44-APC (IM7)	eBioscience	Cat#17-0441-81; RRID:AB_469389
CD62L-PE (MEL-14)	Biolegend	Cat#104408; RRID:AB_313095
ICOS-APC (7E.17G9)	eBioscience	Cat#17-9942-82;RRID:AB_2716948
KLRG1-APC (2F1)	eBioscience	Cat#17-5893-81;RRID:AB_469468
CD69-APC (H1.2F3)	Biolegend	Cat#104513; RRID:AB_492844
Helios-PE/Cy7 (22F6)	eBioscience	Cat#25-9883-41;RRID:AB_2637135
CD137-PE/Cy7 (17B5)	eBioscience	Cat#25-1371-80;RRID:AB_2573397
TLR1-PE (eBioTR23(TR23))	eBioscience	Cat#12-9011-80; RRID:AB_657857
TLR2-PE (6C2)	eBioscience	Cat#12-9021-82;RRID:AB_466230
TLR4-PE (UT41)	eBioscience	Cat#12-9041-80;RRID:AB_466236
TLR5-AF647 (ACT5)	Biolegend	Cat#148103; RRID:AB_2563694
TLR6-PE	R&D Systems	Cat#FAB1533P;RRID:AB_1964728
CD5-PE/Cy7 (53-7.3)	Biolegend	Cat# 100622;RRID:AB_2562773
CD1d-PE (1B1)	eBioscience	Cat#12-0011-82;RRID:AB_465483
CD19-FITC (6D5)	Biolegend	Cat#115506; RRID:AB_313641
Foxp3-APC (FJK-16 s)	eBioscience	Cat#17-5773-82; RRID:AB_469457
Foxp3-PE (FJK-16 s)	eBioscience	Cat#12-5773-82; RRID:AB_465936
Foxp3-eFluor 450 (FJK-16 s)	eBioscience	Cat#48-5773-80; RRID:AB_1518813
IFN-γ-APC (XMG1.2)	eBioscience	Cat#17-7311-81; RRID:AB_469503
IL-17A-FITC (TC11-18H10.1)	Biolegend	Cat#506908; RRID:AB_536010
IL-4-PE (11B11)	Biolegend	Cat#504103; RRID:AB_315317
IL-10-APC (JES5-16E3)	eBioscience	Cat#17-7101-82; RRID:AB_469502
CD45.1-FITC (A20)	Biolegend	Cat#110705; RRID:AB_313494
CD45.2-PerCP/Cy5.5 (104)	Biolegend	Cat#109827; RRID:AB_893352
BrdU-FITC (3D4)	Biolegend	Cat#364103; RRID:AB_2564480
Phospho-IkB alpha (Ser32, Ser36)	eBioscience	Cat#50-9035-42; RRID:AB_2574311
PD-1-FITC (J43)	eBioscience	Cat#11-9985-81; RRID:AB_465471
CXCR5-PE (SPRCL5)	eBioscience	Cat#12-7185-80; RRID:AB_11218887
CD95-AF488 (APO-1/FAS)	eBioscience	Cat#53-0951-82; RRID:AB_10671269
GL7-APC (GL7)	Biolegend	Cat#144617; RRID:AB_2800674
CD138-PerCP/Cy5.5 (281-2)	Biolegend	Cat#142509; RRID:AB_2561600
CD80-APC (16-10A1)	Biolegend	Cat#104714; RRID:AB_313135
CD73-PE/Cy7 (eBioTY/11.8(TY/11.8))	eBioscience	Cat#25-0731-80; RRID:AB_10870789
Chemicals, peptides, and recombinant proteins
Recombinant Human TGF-β1	PeproTech	100-21
Recombinant Murine IL-2	PeproTech	212-12
SYBR Green	Takara	RR420DS
Phorbol 12-myristate 13-acetate (PMA)	Sigma	P8139
Ionomycin	Sigma	I0634
Brefeldin A	eBioscience	00-4506-51
Pertussis Toxin	Merck Millipore	516562-50UG
NP-OVA (Ovalbumin)	Santa Cruz	Sc-396355
Ultra-LEAF-anti-mouse CD28 (37.51)	Biolegend	102115
Ultra-LEAF anti-mouse CD3ε (145-2C11)	Biolegend	100339
DynabeadsTM mouse T-activator CD3/CD28	Thermo	11452D
Critical commercial assays
Naïve CD4+ T Cell Isolation Kit	Miltenyi Biotec	130-104-453
CD4+CD25+ Regulatory T Cell Isolation Kit	Miltenyi Biotec	130-091-041
Foxp3 Staining Buffer Set	eBioscience	00-5523-00
FITC BrdU Flow Kit	BD Pharmingen	559619
Cell Trace Violet Cell Proliferation Kit	Invitrogen	C34571
Deposited data
RNA-seq data	This study	GSE154174
Experimental models: Organisms/strains
Mouse: Foxp3-GFP-hCre	Zhou et al., 2009	N/A
Mouse: Foxp3-GFP KI	Zhou et al., 2009	N/A
Mouse: Lyntm1Sor/J (C57BL/6J)	Jackson Laboratory	003515
Mouse: Tlr2–/– (C57BL/6)	Shanghai Model Organisms	NM-KO-18026
Mouse: Myd88fl/fl	Jackson Laboratory	00-8888
Mouse: CD45.1 (C57BL/6)	Shanghai Model Organisms	NM-KI-210226
Mouse: Rag2–/– (C57BL/6)	Shanghai Model Organisms	NM-KO-190429
Mouse: MRL/lpr	Shanghai Laboratory Animal Center (SLAC) Co., Ltd.	N/A
Oligonucleotides
MOG35-55 (MEVGWYRSPFSRVVHLYRNGK)	GenScript	N/A
All primers used in this study were listed in Table S1.	This study	N/A
Recombinant DNA
Plasmid: pCMV-FLAG-TLR1	GeneCopoeia	N/A
Plasmid: pCMV-HA-TLR2	GeneCopoeia	N/A
Software and algorithms
Prism	GraphPad	https://www.graphpad.com/scientificsoftware/prism/
FlowJo	BD	https://www.flowjo.com/solutions/flowjo