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. 2021 Nov 14;24(12):103454. doi: 10.1016/j.isci.2021.103454

Figure 1.

Figure 1

eIF2B binds GTP

(A) Purified eIF2B (Figure S1A) binds radiolabeled GTP in a concentration-dependent manner. Increasing concentrations of eIF2B bind 32PGTP; BSA was used as a control.

(B) GTP binding by eIF2B increases over time. Error is shown as standard error of the mean (s.e.m).

(C) Both eIF2Bγ and ε are required for binding of GTP. GTP binding is expressed relative to eIF2B complexes (12 μmol of each protein used), and error is shown as s.e.m of ≥3 technical replicates.

(D) Competition binding assays with indicated excess concentrations of unlabeled ATP or GTP. Error as panel B.

See also Figure S1.