Figure 3.

Mutations in the potential GTP-binding pocket of eIF2Bγ impact cell growth and stress responses

(A) Structural alignment of GDP-mannose pyrophosphorylase bound to GTP (2X60, salmon) with yeast eIF2Bγ (6QG0f, green) or eIF2Bε (6QG0j, blue) showing modeled contacts with GTP (red highlighted residues). (Top) Overview; (middle) GTP pocket detail showing eIF2Bγ and GTP only; and (bottom) protein surface rendering. Structural analysis and images used UCSFChimera software version 1.15 (Pettersen et al., 2004).

(B) Plasmid shuffle removing the URA3 GCD1 plasmid using 5FOA leaving the mutated GCD1 as the sole version of eIF2Bγ. SD plates without 5FOA are shown for control.

(C) Growth of strains ±3AT.

See also Figures S3 and S4.